Safety Testing Department

Safety Testing Department

We introduce facilities for safety and usefulness testing as well as introducing cosmetic ingredients. We propose diverse test facilities, ranging from safety testing in wide areas of cosmetics, cosmetic ingredients, quasi drugs, foods and functional materials, to introducing usefullness testing facilities (human in vivo testing/in vitro testing).

Safety Testing Department

Features of Matsumoto Trading Co., Ltd.’s safety and efficacy testing services

Features of Matsumoto Trading Co., Ltd.’s safety and efficacy testing services
Based on our track record and experience we have established for over 360 years of operation as a specialized trading company, we propose multiple test facilities in accordance with customer needs. We introduce test facilities in Japan and other countries, tests conforming to Japanese standards, and tests which comply with overseas standards. We are also ready to propose tests and test facilities in a wide range of fields, including not only cosmetics and cosmetic ingredients but also quasi drugs, foods and functional materials.

  • Human safety confirmation testHuman safety confirmation test
  • In vitro safety testIn vitro safety test
  • Testing for application of quasi drugs and new cosmetic additivesTesting for application of quasi drugs and new cosmetic additives
  • Preservatives-effectiveness test (challenge tests)Preservatives-effectiveness test (challenge tests)
  • Human usefulness evaluation testHuman usefulness evaluation test
  • Evaluation of the Static Sun Protection Factor of Sunscreen / UVA Protection Determination / Evaluating sun product water resistanceEvaluation of the Static Sun Protection Factor of Sunscreen / UVA Protection Determination / Evaluating sun product water resistance
  • In vitro efficacy testIn vitro efficacy test
  • Other usefulness testOther usefulness test

Safety confirmation test

Safety confirmation test

Human safety confirmation test
Human testing for final confirmation of safety of a product, such as cosmetics and quasi drug, is essential in product development. We propose a wide range of tests, from standard tests including human patch tests, RIPT, stinging tests, comedogenicity evaluation and tests for infants and people with atopy including those diagnosed by a dermatologist.

Click here for the list of tests. 

Human safety confirmation test
Human patch test (nomal/sensitive skin)
Repeated insult patch test (RIPT)
Stinging test
Evaluation of comedogenic potential test
Phototoxicity test
Photoallergy test
Safety confirmation test for infants
Safety confirmation test for people with atopy

**Other tests**
A test product is used by test subjects for a specific period with a method specified by the customer, and a dermatologist checks that no skin problems have occurred. We can also conduct questionnaire surveys to learn how it feels to use the product, its perceived effects, and other information. We adjust the number of test subjects and period of use as requested by each customer.

**We can help you recruit test subjects.**
We can also send test subjects to specific facilities, give them explanations about the test, and obtain their consent, so that the customer is able to only concentrate on measurement.

in vitro safety test

in vitro safety test
We are ready to introduce customers to a range of alternatives to animal testing,including tests using cultured cells. We conduct tests in accordance with each guideline at test facilities in a range of countries in addition to Japan.

Click here for the list of tests. 

※The table can be viewed by scrolling left and right.

In vitro safety test Test method Guidelines
Alternative method for skin primary irritation test In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method OECD TG 439
Alternative method for skin sensitization test DPRA : Direct Peptide Reactivity Assay OECD TG 442C
ADRA : Amino acid Derivative Reactivity Assay OECD TG 442C
KeratinoSens™ OECD TG 442D
h-CLAT : human Cell Line Activation Test OECD TG 442E
SENS-IS
Alternative method for phototoxicity test 3T3-NRU phototoxicity test OECD TG 432
Alternative method for eye irritation test SIRC-STE eye irritation test OECD TG 491
Reconstructed human cornea-like epithelium (RhCE) test method for identifying chemicals OECD TG 492
Eye irritation study with isolated bovine corneas OECD TG 437
HET-CAM test
Genotoxicity test Bacterial reverse mutation test (Ames test) OECD TG 471
Chromosomal aberration test using cultured mammalian cells OECD TG 473
In vitro micronucleus test using mammalian cells OECD TG 487

Testing for quasi-drug and new cosmetic additive approval applications

Testing for quasi-drug and new cosmetic additive approval applications
In addition to conducting tests that conform to the GLP based on guidelines for quasi-drugs, we comply with a range of other guidelines. Working together with test facilities, we propose test designs that are customized to the customer’s needs.

Click here for the list of tests. 

Testing for quasi-drug and new cosmetic additive approval applications
Single-dose toxicity test
Primary skin irritation test
Cumulative skin irritation test
Skin sensitization test
Phototoxicity test
Photoallergy test
Eye irritation test
Genotoxicity test
Human patch test

Preservatives-effectiveness tests (challenge tests)

Preservatives-effectiveness tests (challenge tests)
We also accept requests for bactericidal efficacy testing and MIC testing.

Click here for more information

Human usefulness test

Human usefulness test

Human usefulness evaluation test
Working together with test facilities, we customize tests to the customer’s needs and propose schedules and costs.

Click here for the list of tests. 

Human usefulness evaluation test
≫ Evaluation of anti-wrinkle
Moisturizing test
Skin brightening test: Test for blotches (sunspots)
Pigmentation withdrawal test
Hair growth evaluation test (evaluation using phototrichogram/visual judgment of grade, etc.)
Test evaluating efficacy in treating skin roughness
Lasting test
Evaluation test for face lifts
Pore evaluation test (pore opening, etc.)
Permeability test (tape stripping method)
Ultrasound image evaluation with DermaScan® (Integral Corporation)
Skin flora test
Evaluation test of the use of beauty equipment by skin measurement

Evaluation of the Static Sun Protection Factor of Sunscreen / UVA Protection Determination / Evaluating sun product water resistance

Evaluation of the Static Sun Protection Factor of Sunscreen / UVA Protection Determination / Evaluating sun product water resistance
We can suggest several testing laboratories that can perform Evaluation of the Static Sun Protection Factor of Sunscreen / UVA Protection Determination /Evaluating sun product water resistance based on the latest ISO guidelines.

in vitro efficacy test

in vitro efficacy test
We can propose usefulness tests to evaluate whitening, anti-wrinkle, moisturizing, anti-inflammation, anti-oxidation, hair growth, etc. using cell culture and biochemical methods. We can also propose domestic and overseas testing institutes that conduct tests other than those listed, so please feel free to contact us for more information.

Click here for the list of tests. 

※The table can be viewed by scrolling left and right.

Category in vitro efficacy test Keywords
Enzymes Tyrosinase activity inhibition test Skin brightening
Cell B16 mouse melanoma cell test Skin brightening
Cell 3D-model skin-brightening efficacy test Skin brightening
Cell Collagen production test Anti-wrinkle, moisturizing
Cell Hyaluronic acid production test Anti-wrinkle, moisturizing
Enzymes Elastase activity inhibition test Anti-wrinkle, moisturizing
Enzymes Collagenase activity inhibition test Anti-wrinkle, moisturizing
Enzymes Hyaluronidase activity inhibition test Anti-wrinkle, anti-inflammatory
Enzymes Test measuring superoxide dismutase-like activity Antioxidant
Enzymes Test measuring DPPH radical scavenging ability Antioxidant
Enzymes Test measuring anti-glycation activity Anti-glycation
Cell Hair papilla cell test Hair growth
Enzymes 5α-reductase inhibition test Hair growth
Cell Cell activation test (epidermal keratinocytes cells, fibroblasts cells, hair papilla cells) Various
Cell Various related gene expression analysis (moisturizing, barrier function, anti-wrinkle, hair growth, etc.) Various
Cell Exhaustive gene expression analysis Various

Other usefulness tests

Other usefulness tests
We also contribute to the fields of preventive medicine, foods and functional materials to increase the health and beauty of people from inside the body. We also support customers in obtaining the data needed to submit notifications of food with functional claims. We also conduct tests other than those listed below. Please feel free to contact us for inquiries.

Click here for the list of tests. 

※The table can be viewed by scrolling left and right.

Test name Outline
Observation of skin penetration kinetics using PM-SRS Skin penetration kinetics observation technology utilizing phase modulation induced Raman scattering (PM-SRS) is used to confirm the permeability of various components in 3D cultured skin by directly observing and imaging the substances of interest.
Skin penetration test using Franz diffusion cell The permeability of the test substance is evaluated using artificial membranes, cultured skin, or human explanted skin in the diffusion cell.
Biodegradability test We evaluate the biodegradability of cosmetic formulations as well as containers. Test methods include OECD TG301F.
Transmittance Measurement Test Measure the transmittance of wavelengths in the blue light and near-infrared regions.
Tests related to PM 2.5 Black iron oxide particles (particle diameter: 1 μm) are regarded as *PM 2.5.
After applying the test product to the bioskin plate, iron particles are adhered to the bioskin plate; iron is measured using an ICP emission spectrometer.
Pollen release test After pollen is applied to the test specimen, the pollen is released by applying a specified amount of vibration (gentle swipe by hand). Count the pollen remaining on the specimen.
Mosquito repellence test Aedes albopictus mosquitos are released in a mesh cage. Then, test is conducted by inserting arms coated with the test product into the cage.
Various types of hair tests Taking photographs of hair surface/hair friction test/hair bending test/hair tensile strength test/hair gloss test/hair moisture test, etc.
Moisturizing test using a supplement
Test of the efficacy of supplement intake in the alleviation of knee osteoarthritis
Test of the efficacy of supplement intake for hair growth
Test of the efficacy of beautifying skin through the intake of beauty beverages
Test to confirm anti-stress effects
Test of the efficacy of massage for blood flow improvement
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